We have sequenced, assembled and annotated the genome of Clostridium sordellii strain E204.. Paraclostridium sordellii strain:E204

Clostridium sordellii is an anaerobic bacterium associated with rare but severe infections of man and animals. Human infections are associated with trauma, toxic shock and often-fatal gynaecological infections. Some strains are known to produce the large clostridial cytotoxins TcsH and TcsLH, relatives of the cytotoxins found in C. difficile, C. novyi and C. perfringens. We have undertaken a genomic analysis of a collection of C. sordellii strains derived from cases of clinical or veterinary disease. Phylogenetic analysis reveals a highly genetically diverse species. The majority of strains do not encode tcsL or tcsH, but all strains encode the putative virulence factors sordellilysin, phospholipase C and neuraminidase. Where tcsL or tcsH are present, these are localised on a plasmid. Large scale sequencing on this neglected pathogen has to date been limited, these genomes will facilitate further research on this important pathogen.

索氏梭菌（Clostridium sordellii）是一类厌氧菌，可引发人和动物罕见但病情危重的感染。人类感染病例多与创伤、中毒性休克以及常可致命的妇科感染相关。部分菌株可产生大型梭菌毒素TcsH与TcsLH，这类毒素与艰难梭菌（Clostridium difficile, C. difficile）、诺维氏梭菌（Clostridium novyi, C. novyi）及产气荚膜梭菌（Clostridium perfringens, C. perfringens）所携带的毒素为同源类似物。本研究对一批从临床或兽医病例中分离得到的索氏梭菌菌株开展了基因组分析。系统发育分析结果显示，该物种具有高度的遗传多样性。多数菌株不编码tcsL或tcsH基因，但所有菌株均编码推定毒力因子索氏溶素（sordellilysin）、磷脂酶C（phospholipase C）以及神经氨酸酶。若菌株携带tcsL或tcsH基因，则这些基因定位于质粒之上。迄今为止，针对这一被忽视的病原菌的大规模测序工作仍较为有限，本次获得的基因组数据将有力推动该重要病原菌的后续研究。