Additional file 5 of Genome data uncover four synergistic key regulators for extremely small body size in horses

Functional classification of genes. Variants identified in ROH regions in the two Miniature Shetland ponies and one standard sized Shetland pony, which were estimated to be deleterious (SIFT prediction) were investigated for their genomic positions in genes. In addition, variants, which showed an exclusively homozygous mutant genotype in all three Shetland ponies, were added to the analysis. Human orthologue genes were functionally classified using DAVID Functional Annotation Tool. (XLSX 23 kb)

基因功能分类分析。对2匹迷你设得兰矮种马（Miniature Shetland ponies）与1匹标准型设得兰矮种马的纯合子区域（Runs of Homozygosity, ROH）内鉴定得到的、经SIFT（Sorting Intolerant From Tolerant）预测为有害的变异展开研究，明确其在基因中的基因组定位。此外，将在全部3匹设得兰矮种马中仅呈现纯合突变基因型的变异纳入本次分析。利用DAVID功能注释工具（DAVID Functional Annotation Tool）对人类同源基因进行功能分类。（XLSX 23 KB）