The profile of exosomal and cellular miRNA in ovarian cancer cell lines

To check the profile of exosomal and cellular miRNA in ovarian cancer cell lines, total RNA were extracted from exosomes and cells. Thirteen ovarian cancer cell lines (A2780, ES-2, CAOV3, SKOV3, OV-90, OAW42, MCAS, COV362, RMG-1, RMUG-S, KURAMOCHI, NIH-OVCAR3 and A2780cis) were investigated, and HOSE1, HOSE2 and HOSE3 (human ovarian surface epithelim cell lines) were used as control. To check the profile of exosomal and cellular miRNA in ovarian cancer cell lines, total RNA were extracted from exosomes and cells. Microarray analysis was performed using 50 ng of exosomal miRNAs and 100 ng of cellular miRNAs.

为探究卵巢癌细胞系中外泌体与细胞内微小RNA (miRNA) 的表达谱，研究人员从外泌体及细胞中提取总RNA。本研究共纳入13株卵巢癌细胞系（A2780、ES-2、CAOV3、SKOV3、OV-90、OAW42、MCAS、COV362、RMG-1、RMUG-S、KURAMOCHI、NIH-OVCAR3及A2780cis）进行检测，并以HOSE1、HOSE2、HOSE3三株人卵巢表面上皮细胞系作为对照。为分析卵巢癌细胞系中外泌体与细胞内微小RNA的表达谱，研究人员再次从外泌体及细胞中提取总RNA。随后采用50 ng外泌体来源微小RNA与100 ng细胞内来源微小RNA进行基因芯片分析。