Transcriptome analysis of lung neutrophils from mice infected with Mycobacterium tuberculosis

Neutrophils are accumulated in mouse lungs following Mycobacterium tuberculosis infections. Increased influx of neutrophils is associated with increased susceptibility to disease, but the underlying mechanisms of neutrophil mediated Tuberculosis disease pathogenesis is poorly understood. To understand the transcriptomic differences between resistant (C57 BL/6) and susceptible (C3HeB/FeJ) mouse lung neutrophils, we sorted them at Day 29 post infection and analyzed them by bulk mRNA sequencing. Bulk mRNA sequencing to study transcriptomic differences in neutrophils at Day 29 post infection with Mycobacterium tuberculosis HN878 from C57 BL/6 and C3HeB/FeJ mouse lungs.

结核分枝杆菌（Mycobacterium tuberculosis）感染小鼠后，中性粒细胞（Neutrophils）会在肺部募集。中性粒细胞浸润增多与疾病易感性升高相关，但中性粒细胞介导的结核病（Tuberculosis）发病机制仍未得到充分阐明。为解析抗结核品系（C57 BL/6）与易感品系（C3HeB/FeJ）小鼠肺部中性粒细胞的转录组差异，本研究于感染结核分枝杆菌HN878后第29天对中性粒细胞进行分选，并通过批量mRNA测序（bulk mRNA sequencing）开展分析。