Data for: Evaluation of transcriptional biomarkers using a high-resolution regression approach: Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)

Data for the paper 'Evaluation of transcriptional biomarkers using a high-resolution regression approach: Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)'.

ABSTRACT: We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13–1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold increases within the exposure range, high Cu concentrations were required (38–160 µg/L). Although gill responses were generally more robust compared to digestive glands, overall small response magnitudes and moderate sensitivity may set limit for potential application as general biomarkers of chemical stress.

Full reference:
Ekelund Ugge, G.M.O., Jonsson, A., Walstad, A., Berglund, O. (2022). Evaluation of transcriptional biomarkers using a high-resolution regression approach: Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina). Environ Toxicol Pharmacol, 90, 103795.
https://doi.org/10.1016/j.etap.2021.103795

本数据集关联论文《采用高分辨率回归方法评估转录生物标志物：铜暴露淡水贻贝（Anodonta anatina）中选定转录本的浓度依赖性》。

摘要：本研究针对选定的基因转录本（cat、gst、hsp70、hsp90、mt及sod）的浓度依赖性开展测试，以评估其作为化学胁迫生物标志物的潜力。与常规采用因子设计及少量暴露浓度的研究思路不同，本研究针对高分辨率浓度梯度进行回归分析。具体而言，本研究将淡水贻贝（Anodonta anatina）进行96小时急性铜暴露，设置13个标称浓度（实测浓度范围0.13~1600 μg/L），并通过逆转录定量实时聚合酶链反应（RT-qPCR）对转录本进行定量检测。在消化腺中，cat、hsp90及mt的表达量随水体铜浓度升高而下调（p < 0.05），但响应幅度在下调小于2倍时即趋于饱和。在鳃组织中，gst、hsp70、hsp90及mt的表达量随水体铜浓度升高而上调（p < 0.05）。尽管在暴露浓度范围内，hsp70、hsp90及mt的表达上调幅度可超过2倍，但需达到较高的铜浓度（38~160 μg/L）方可实现。尽管鳃组织的响应总体上较消化腺更为显著，但整体响应幅度较小且灵敏度中等，这可能限制了其作为通用化学胁迫生物标志物的应用潜力。

完整参考文献：Ekelund Ugge, G.M.O.、Jonsson, A.、Walstad, A.、Berglund, O.（2022）。采用高分辨率回归方法评估转录生物标志物：铜暴露淡水贻贝（Anodonta anatina）中选定转录本的浓度依赖性。《环境毒理学与药理学（Environ Toxicol Pharmacol）》，90卷，103795。https://doi.org/10.1016/j.etap.2021.103795