MOESM8 of Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

Additional file 8. Glycosyl residue composition and total carbohydrate of cell wall extracts from stem tissues of 9-month-old, greenhouse-grown P. deltoides WT and PtGAUT12.1-OE lines (AB29.2, AB29.7, and AB29.12). Data are average μg amount of each designated monosaccharide per mg alcohol insoluble residue (AIR), measured by GC–MS of tetramethylsilane (TMS) derivatives. Wall extracts are from sequential extractions of AIR using 50 mM ammonium oxalate, 50 mM sodium carbonate, 1 M KOH, 4 M KOH, 100 mM sodium chlorite (chlorite), 4 M KOH post-chlorite (PC), and the final residual insoluble pellet. Values in bold are those of the transgenic lines that are significantly different from WT values at *P < 0.05 and **P < 0.001 significant levels (one-way ANOVA followed by Tukey’s multiple comparison test).

补充文件8。9月龄温室栽培的美洲黑杨（P. deltoides）野生型（WT）及PtGAUT12.1过表达（OE）株系（AB29.2、AB29.7和AB29.12）的茎组织细胞壁提取物的糖基残基组成与总碳水化合物含量。数据为每毫克醇不溶残渣（alcohol insoluble residue, AIR）中各指定单糖的平均微克数，通过气相色谱-质谱联用（gas chromatography-mass spectrometry, GC-MS）分析四甲基硅烷（tetramethylsilane, TMS）衍生物进行测定。细胞壁提取物通过对醇不溶残渣进行连续萃取获得，依次使用50 mM草酸铵、50 mM碳酸钠、1 M氢氧化钾、4 M氢氧化钾、100 mM亚氯酸钠（亚氯酸钠）、亚氯酸钠处理后再用4 M氢氧化钾萃取（PC），以及最终残留的不溶性沉淀。加粗数值为转基因株系的测定值，其与野生型株系的对应数值存在显著统计学差异：*P < 0.05，**P < 0.001（采用单因素方差分析（one-way ANOVA）后进行Tukey多重比较检验）。