Identification of metastasis-related genes and metastasis-related pathway in squamous cell carcinoma

To explore metastasis-related genes in high-metastatic cells (HOC313-LM), we performed gene expression array analysis with HOC313-LM and HOC313-Parent (HOC313-P). Moreover, to identify metastasis-related signaling pathway, gene expression profiles of each of transplanted tumor tissues in the primary site or in the metastatic site were analyzed by systems biology analysis and Ingenuity Pathway Analysis (IPA; Ingenuity Systems, Inc., Redwood City, CA). As a result, RhoA signaling pathway emerged as the critical molecular pathway in the metastatic tumor compared to the primary tumor. Identification of metastasis-related genes and metastasis-related pathway in squamous cell carcinoma Extraction of difference of gene expression level between high-metastatic subline (HOC313-LM) and low-metastatic subline (HOC313-P)

为探究高转移细胞（HOC313-LM）中的转移相关基因，本研究针对HOC313-LM及亲本细胞系HOC313-Parent（简称HOC313-P）开展了基因表达芯片分析。此外，为鉴定转移相关信号通路，本研究通过系统生物学分析与IPA（Ingenuity Pathway Analysis，Ingenuity Systems公司，美国加利福尼亚州雷德伍德城），对原位移植瘤组织及转移灶移植瘤组织的基因表达谱进行了分析。结果显示，相较于原位肿瘤，RhoA信号通路是转移性肿瘤的关键分子通路。本研究旨在鉴定鳞状细胞癌中的转移相关基因与通路，并提取高转移亚系（HOC313-LM）与低转移亚系（HOC313-P）之间的基因表达水平差异。