RNA sequencing of HCC cells after endoplasmic reticulum stress induced by tunicamycin. RNA sequencing of HCC cells after endoplasmic reticulum stress induced by tunicamycin

In order to explore the role of ER stress in the development and progression of HCC, RNA sequencing was performed to detect the differential RNA levels before and after ER stress, thus providing strong evidence for the search of ER stress-related genes. Overall design: We treated HepG2 cells with tunicamycin for 24 h and then extracted total RNA for detection.

为探究内质网应激（ER stress）在肝细胞癌（HCC）发生发展中的作用，本研究通过RNA测序（RNA sequencing）检测内质网应激处理前后的差异RNA表达水平，为内质网应激相关基因的筛选提供有力依据。实验设计概述：我们使用衣霉素（tunicamycin）处理HepG2细胞24小时，随后提取总RNA进行检测。