Data_Sheet_1_A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury.pdf

We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum, namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe2+ level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.

本研究从川明参（Chuanminshen violaceum）的根部分离纯化得到一种新型多糖，命名为川明参多糖（CVP），并确证了其结构与分子量。进一步通过体内实验证实，CVP对小鼠心肌缺血再灌注（I/R）损伤具有保护作用：可显著缩小I/R诱导的心肌梗死（MI）面积、降低心脏损伤生物标志物的分泌水平，并改善心功能。随后，本研究构建心肌缺氧再复氧（A/R）损伤模型以模拟再灌注损伤。值得注意的是，铁死亡是A/R损伤后H9c2细胞的主要死亡方式。与此同时，CVP可通过降低细胞内Fe²+水平、上调谷胱甘肽过氧化物酶4（GPX4）的表达、抑制脂质过氧化，从而显著抑制铁死亡以对抗A/R损伤。综上，结构明确的CVP可通过抑制铁死亡改善心肌缺血再灌注损伤。