Additional file 1: of Guide for library design and bias correction for large-scale transcriptome studies using highly multiplexed RNAseq methods
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https://springernature.figshare.com/articles/Additional_file_1_of_Guide_for_library_design_and_bias_correction_for_large-scale_transcriptome_studies_using_highly_multiplexed_RNAseq_methods/9600224/1
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Sample script. This script demonstrates the library bias correction by NBGLM-LBC, performs the simulation experiments for Fig. 3, and draws Figs. 1b, 2a, c and 3 bottoms. Before running this script using R, LBC-additionalFile2.txt (Additional file 2) and LBC-additionalFile3.txt (Additional file 3) must be placed within the working directory, and the preprocessCore, DESeq2 and factoextra packages must be installed. (R 10 kb)
示例脚本。本脚本用于演示NBGLM-LBC实现的文库偏倚校正功能,开展图3对应的仿真实验,并绘制图1b、图2a、图2c以及图3的下半部分子图。在使用R运行该脚本前,需将LBC-additionalFile2.txt(附加文件2)与LBC-additionalFile3.txt(附加文件3)放置于工作目录中,同时需提前安装preprocessCore、DESeq2及factoextra这三个R包。(基于R环境,大小10 KB)
提供机构:
figshare
创建时间:
2019-08-14



