Gene expression analysis of B cell differentiation using B cell blasts and developmentally arrested plasmablasts. Mus musculus

In vitro generated blasts from B cells deficient in prdm1 were isolated according to whether they were attempting to express Blimp1, reported by a GFP cassette knocked into the prdm1 locus Overall design: After four days stimulation with CD40L, IL4 and IL5, cells were FACS sorted into GFP+ and GFP- populations, RNA extracted and gene expression analysed by microarray. Data presented in Peparzak et al, 2013, Fig 3b.

从prdm1（PR结构域锌指蛋白1）缺陷型B细胞体外诱导生成的母细胞，依据其是否正在表达Blimp1（B淋巴细胞诱导成熟蛋白1）完成分离；该Blimp1的表达状态由敲入prdm1基因座的GFP（绿色荧光蛋白）标签盒进行示踪报告。
实验设计概述：使用CD40L、IL4（白细胞介素4）与IL5（白细胞介素5）刺激细胞四天后，通过FACS（荧光激活细胞分选术）将细胞划分为GFP阳性与GFP阴性两个群体，随后提取总RNA并通过微阵列（基因芯片）分析基因表达水平。
本数据集相关数据发表于Peparzak等人2013年的研究，对应其中的图3b。