Identification of HOTAIR target genes using RNA-seq after overexpression (OE) and downregulation (si) of HOTAIR in human adipose derived stem cells isolated from abdominal (ABD) and gluteofemoral (GF) subcutaneous adipose tissue. Identification of HOTAIR target genes using RNA-seq after overexpression (OE) and downregulation (si) of HOTAIR in human adipose derived stem cells isolated from abdominal (ABD) and gluteofemoral (GF) subcutaneous adipose tissue

Human adipose serived stem cells (ASC) isolated from A and GF adipose tissue obtained from premenopausal women were used to overexpress and downregulate HOTAIR (respectively). RNA-seq was used to study the transcriptome of control and treated cells. Overall design: RNA-seq was used to study the transcriptome of control and treated cells.

本研究使用分离自绝经前女性获取的A组与GF组脂肪组织的人脂肪来源干细胞（Human adipose derived stem cells，ASC），分别对其进行HOTAIR的过表达与下调实验。采用RNA测序（RNA-seq）技术对对照组与处理组细胞的转录组进行研究。实验整体设计：采用RNA测序技术对对照组与处理组细胞的转录组进行研究。