UUO-rUUO small RNA seq, Denby lab 2019

Small RNA sequencing of sorted cell type, mice kidneys, after unilateral ureteral obstructive (UUO) surgery after 2 days, 7 days and a reversible (rUUO) 14 day model with corresponding sham operated mice. The goal of the study is to identify micro-RNA dysregulation of expression between different cell types corresponding with kidney injury, in order to identify and assess biomarkers which could be translated into the clinical setting. Each group was sequenced from 4 mice, and 4 cell types. 2 day UUO kidneys were sorted into PDGFRβ+, F480+, LTL+ and CD31+ cell types before the RNA was extracted and small RNA sequenced for 4 mice. The same protocol was followed for 7 day UUO mice, rUUO mice, as well as sham operated mice for reference.

针对单侧输尿管梗阻（unilateral ureteral obstructive, UUO）术后2天、7天以及可逆性单侧输尿管梗阻（reversible UUO, rUUO）术后14天的小鼠肾脏分选细胞类型开展小RNA测序，同步设置对应假手术小鼠作为对照。本研究旨在鉴定与肾损伤相关的不同细胞类型间的microRNA (miRNA) 表达失调情况，以识别并评估可转化至临床应用场景的生物标志物。本研究每组均使用4只小鼠的样本，涵盖4种细胞类型：针对术后2天的UUO模型小鼠肾脏，先将其分选为PDGFRβ+、F480+、LTL+及CD31+四种细胞类型，随后提取RNA并进行小RNA测序，每组均采用4只小鼠的样本。术后7天UUO模型小鼠、rUUO模型小鼠以及作为对照的假手术小鼠均遵循相同实验流程。