Interaction of a flavone loaded on surface-modified dextran-spooled superparamagnetic nanoparticles with β-cyclodextrin and DNA

Flavones are biologically active compounds obtained mainly from plant sources. Pharmaceutically important compounds can be delivered to the physiological target by loading them in carriers like cyclodextrins and magnetic nanoparticles. Herein, the binding of 6-methoxyflavone to β-cyclodextrin and DNA is studied using UV–visible absorption and fluorescence spectroscopy. The loading of 6-methoxyflavone onto a magnetic nanoparticles is employed. β-cyclodextrin encapsulates the 6-methoxyflavone to form an inclusion complex. β-cyclodextrin also used to draw forth 6-methoxyflavone loaded onto a magnetic nanoparticles. The morphology, magnetic property and the crystallite size of the nanoparticles are studied using scanning electron microscopy, vibrating sample magnetometry and X-ray diffraction techniques, respectively. The binding of the drug-loaded magnetic nanoparticles to DNA shows that the compound is accessible to DNA and available mostly on the surface of the nanoparticles despite a modified dextan polymer supposedly encapsulates the flavone.

黄酮类化合物是一类主要源自植物的生物活性化合物。可通过将药用活性化合物负载于环糊精、磁性纳米颗粒等载体的方式，将其递送至生理靶点。本文采用紫外-可见吸收光谱与荧光光谱法，研究了6-甲氧基黄酮（6-methoxyflavone）与β-环糊精以及脱氧核糖核酸（DNA）的结合作用，并开展了将6-甲氧基黄酮负载于磁性纳米颗粒的实验。β-环糊精可包裹6-甲氧基黄酮形成包合复合物，同时亦可用于脱附负载于磁性纳米颗粒的6-甲氧基黄酮。本研究分别采用扫描电子显微镜（SEM）、振动样品磁强计（VSM）以及X射线衍射（XRD）技术，对纳米颗粒的形貌、磁学性能与晶粒尺寸进行了表征。结果表明，尽管据称改性右旋糖酐聚合物包裹了该黄酮类化合物，但载药磁性纳米颗粒与DNA的结合实验显示，该化合物可与DNA接触，且主要分布于纳米颗粒表面。