Differentiating diploid and triploid individuals using single nucleotide polymorphisms genotyped by amplicon-sequencing

Triploidy can occur naturally or be induced in fish and shellfish during artificial propagation in order to produce sterile individuals. Fisheries managers often stock these sterile triploids as a means of improving angling opportunity without risking unwanted reproduction of the stocked fish. Additionally, the rearing of all-triploid individuals has been suggested as a means to reduce the possibility of escaped aquaculture fish interbreeding with wild populations. Efficient means of determining if an individual is triploid or diploid are therefore needed both to monitor the efficacy of triploidy inducing treatments and, when sampling fish from a body of water that has a mixture of diploids and triploids, to determine the ploidy of a fish prior to further analyses. Currently, ploidy is regularly measured through flow cytometry, but this technique typically utilizes a fresh blood sample. This study presents an alternative, cost-effective method of determining ploidy by analyzing amplicon...

三倍体（Triploidy）可自然发生，也可在鱼类与甲壳贝类的人工繁育过程中诱导产生，以获得不育个体。渔业管理者常投放此类不育三倍体，在规避养殖鱼类意外繁殖风险的同时，提升垂钓机会。此外，全三倍体个体的养殖被认为可降低逃逸养殖鱼类与野生种群杂交的可能性。因此，亟需高效的个体倍性（ploidy）鉴定手段，既用于监测三倍体诱导处理的效果，也可在从同时混存有二倍体（diploid）与三倍体的水域采样鱼类时，先完成待分析鱼类的倍性鉴定。当前，倍性检测通常采用流式细胞术（flow cytometry），但该技术一般需要新鲜血液样本。本研究提出了一种通过扩增子（amplicon）分析实现倍性鉴定的替代性、高性价比方案