SH1036603.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 为界定、识别、传播以及处理基于DNA的物种假说（DNA-based Species Hypotheses, SH）提供了统一的标准化流程。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer, ITS）序列，均通过应用一组动态距离阈值（0.5%~3.0%）被聚类至近似物种水平。所有物种假说均会被赋予一个以数字对象标识符（Digital Object Identifier, DOI）为形式的唯一且稳定的标识名称，其分类学与生态学注释信息将通过分布式网页端第三方注释工作完成核验。研究团队会整合单个SH内所有序列的鉴定结果，尽可能将该SH与对应分类单元名称及其分类层级（如门、纲、目等）建立关联，并选取一条经自动或人工指定的序列作为该SH的代表序列。此类代表序列已在官方仓库（https://unite.ut.ee/repository.php）对外发布，可供科研社群用于本地序列相似性搜索、高通量测序分析流程等科研场景。随着公开真菌ITS序列数量的持续增长，该系统与配套数据集将自动完成更新。