ADBP-1 regulates ADR-2 nuclear localization to control editing substrate selection

We generated RNA-seq libraries from three biological replicas of adbp-1 mutant worms at the embryo and L4 developmental stages. We wanted to identify editing sites globally in adbp-1 mutant worms and understand the preferable ADR-2 5' and 3' nearest neighbors of editing sites in wild-type worms in contrast to adbp-1 mutant worms. In addition, we wanted to test if the cytoplasmic localization of ADR-2 causes the downregulation of 3'UTR edited genes and lncRNAs, and test the effect of lacking ADBP-1 on global gene expression. Overall design: A-to-I RNA editing detection of known and de-novo editing sites and gene expression analysis in adbp-1 mutant worms.

我们从胚胎发育阶段和L4幼虫发育阶段的adbp-1突变体线虫的3份生物学重复样本中构建了RNA-seq文库。本研究旨在全基因组范围内鉴定adbp-1突变体线虫中的RNA编辑位点，并解析相较于adbp-1突变体线虫，野生型线虫中编辑位点旁侧ADR-2的偏好性5'与3'邻接序列。此外，我们还将验证ADR-2的细胞质定位是否会引发3'非翻译区（3'UTR）编辑基因及长链非编码RNA（lncRNAs）的表达下调，并探究ADBP-1缺失对全基因组基因表达的影响。整体实验设计：对adbp-1突变体线虫中的已知及从头（de-novo）A-to-I RNA编辑位点进行检测，并开展基因表达分析。