Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del/+-Del/+ - 5224_5173

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.

本研究对成对陌生个体间的社交互动与交流行为进行了评估。针对社交行为与超声发声（USVs）的录制工作，我们选择以个体对作为研究对象，原因在于当前无法在动物近距离互动时分辨超声发声的发出个体。因此，我们对来自不同饲养笼、年龄一致（14~20周龄）且基因型相同的两只陌生个体之间的无干扰成对互动，开展了为期47小时（两天两夜）的录制。为此，我们将活体小鼠追踪系统（Live Mouse Tracker，插件版本931）与一支CM16/CMPA麦克风（德国格利尼克Avisoft Bioacoustics公司出品）相连，该麦克风接入Avisoft Ultrasound Gate 416设备，其参数设置为：采样率300 kHz，格式为16位；触发条件：该通道电平；预触发时长1 s；保持时长1 s；有效时长>0.005 s；触发事件：25~125 kHz频段内能量占比2%且熵值<50%；设备由德国格利尼克的Avisoft Bioacoustics公司生产。活体小鼠追踪系统与Avisoft设备系统依据de Chaumont等人2021年发表的实验方案实现同步。每份上传文件均包含来自活体小鼠追踪系统的SQLite数据库（已完成处理并完成超声发声同步），以及一个压缩包，其中包含Avisoft设备自动录制的全部超声发声序列。