Cardiac Sex Differences in the Transcriptome of C57 Male and Female E9.5 Hearts

Purpose: The goals of this study are to compare E9.5 male and female C57 mouse embryonic hearts transcriptome profiling (RNA-seq) to conclude cardiac sex differences at the mRNA level. Methods: mRNA profiles of E9.5 male and female C57 mouse embryonic hearts were generated by deep sequencing, n=4 for each sex, using Illumina HiSeq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to the mouse genome (build mm10) and identified 16,460 transcripts in the mouse hearts. Overall design: E9.5 male and female C57 mouse embryonic hearts RNAseq

研究目的：本研究旨在对比E9.5天雌雄C57小鼠胚胎心脏的转录组表达谱（RNA-seq）数据，以阐明mRNA水平上的心脏性别差异。实验方法：采用Illumina HiSeq2500测序平台，对E9.5天雌雄C57小鼠胚胎心脏的mRNA表达谱进行深度测序，每组设置4个生物学重复（n=4）。对通过质量过滤的序列读段，采用两种方法在转录本异构体水平进行分析：其一为Burrows-Wheeler比对工具（Burrows–Wheeler Aligner, BWA）结合方差分析（ANOVA），其二为TopHat结合Cufflinks。研究结果：通过优化后的数据分析流程，我们将每个样本约3000万条序列读段比对至小鼠基因组（版本mm10），并在小鼠心脏中鉴定出16460个转录本。实验整体设计：针对E9.5天雌雄C57小鼠胚胎心脏开展RNA测序实验。