DataSheet1_Wide-field optical coherence tomography for microstructural analysis of key tissue types: a proof-of-concept evaluation.PDF

Introduction: The presence of positive margins following tumor resection is a frequent cause of re-excision surgery. Nondestructive, real-time intraoperative histopathological imaging methods may improve margin status assessment at the time of surgery; optical coherence tomography (OCT) has been identified as a potential solution but has not been tested with the most common tissue types in surgical oncology using a single, standardized platform.
Methods: This was a proof-of-concept evaluation of a novel device that employs wide-field OCT (WF-OCT; OTIS 2.0 System) to image tissue specimens. Various cadaveric tissues were obtained from a single autopsy and were imaged with WF-OCT then processed for permanent histology. The quality and resolution of the WF-OCT images were evaluated and compared to histology and with images in previous literature.
Results: A total of 30 specimens were collected and tissue-specific microarchitecture consistent with previous literature were identified on both WF-OCT images and histology slides for all specimens, and corresponding sections were correlated. Application of vacuum pressure during scanning did not affect specimen integrity. On average, specimens were scanned at a speed of 10.3 s/cm2 with approximately three features observed per tissue type.
Conclusion: The WF-OCT images captured in this study displayed the key features of the most common human tissue types encountered in surgical oncology with utility comparable to histology, confirming the utility of an FDA-cleared imaging platform. With further study, WF-OCT may have the potential to bridge the gap between the immediate information needs of the operating room and the longer timeline inherent to histology workflow.

引言：肿瘤切除术后切缘阳性是再次手术的常见诱因。非侵入式、实时术中组织病理学成像方法可在手术过程中优化切缘状态的评估；光学相干断层扫描（optical coherence tomography，OCT）被认为是一种潜在解决方案，但目前尚未依托单一标准化平台，针对外科肿瘤学领域最常见的组织类型开展相关测试。
方法：本研究针对一款采用宽场光学相干断层扫描（wide-field OCT，WF-OCT；OTIS 2.0系统）技术的新型设备开展概念验证评估，以对组织标本进行成像。研究采集了单次尸检获取的多具尸体组织，先通过WF-OCT进行成像，随后进行永久性组织学处理。研究人员对WF-OCT图像的质量与分辨率进行评估，并将其与组织学切片及既往文献中的图像进行对比。
结果：本研究共收集30份组织标本，所有标本的WF-OCT图像与组织学切片上均观察到与既往文献一致的组织特异性微结构，且对应切片具有良好的相关性。扫描过程中施加真空压力并未对标本完整性造成影响。标本的平均扫描速度为10.3秒/平方厘米，每类组织平均可观察到约3个特征性结构。
结论：本研究获取的WF-OCT图像可呈现外科肿瘤学中最常见人体组织类型的关键特征，其效用与组织学检查相当，证实了这款获得美国食品药品监督管理局（Food and Drug Administration，FDA）认证的成像平台的实用价值。经进一步研究，WF-OCT或可填补手术室即时信息需求与组织学工作流程固有耗时较长之间的空白。