Proteomic Profiling Identifies Candidate Diagnostic Biomarkers of Hydrosalpinx in Endometrial Fluid

This proteomic profiling study applied sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS) to study hydrosalpinx cyst fluid and pre- and post-salpingectomy endometrial fluid.
In this exploratory pilot study, HCF and EF samples were collected from 10 women with hydrosalpinx, diagnosed according to standard clinical protocol, at the time of sal-pingectomy. In addition, endometrial fluid samples were collected from seven of these women one month after surgery. Samples were not obtained from three participants be-cause they failed to attend the post-surgical examination visit.
The hydrosalpinx cyst fluid was obtained directly from the removed surgical speci-men by puncturing the dilated area of the fallopian tube by aspiration of the contents with a syringe (approx. 200 μL) once out of the patient.
To maximize the number of identified proteins, all HCF and pre- and post-salpingectomy EF samples were pooled (2 μg of each) to build a spectral library con-taining a total of 50 μg of protein extracts for proteomic characterization. Pooled protein extracts were loaded onto a one-dimensional SDS-polyacrylamide gel and separated by electrophoresis to determine the protein profiles of each condition. The gel was digested to construct the library for label-free quantification analysis of the study samples using SWATH-MS.
Once the spectral library of the proteins present in the different types of fluids was generated, the individualized identification of the quantitative profile of each type of fluid was carried out.
RAW data about protein areas, Spectral library, Spectral library search results and Spectral library protein identification are contained in this dataset.

本蛋白质组学表征研究采用全理论碎片离子谱连续窗口采集质谱（SWATH-MS）技术，对输卵管积水囊肿液及输卵管切除术术前、术后的子宫内膜液开展研究。
本探索性预实验中，研究人员于输卵管切除术时，从10名按标准临床流程确诊的输卵管积水患者体内采集了输卵管积水囊肿液（hydrosalpinx cyst fluid, HCF）与子宫内膜液（endometrial fluid, EF）样本。此外，术后1个月，从上述10名患者中的7名体内采集了子宫内膜液样本；剩余3名参与者因未参加术后随访检查，未获取其样本。
输卵管积水囊肿液直接取自切除的手术标本：于患者离体后，用注射器抽吸输卵管扩张部位的内容物（约200 μL）获取样本。
为最大化可鉴定蛋白质的数量，将所有输卵管积水囊肿液及输卵管切除术前后的子宫内膜液样本按每份2 μg进行混合，构建总蛋白提取物为50 μg的谱图库，用于蛋白质组学表征分析。
将混合后的蛋白提取物上样至一维十二烷基硫酸钠-聚丙烯酰胺凝胶（SDS-PAGE），通过电泳分离以明确各分组的蛋白质谱；随后对凝胶进行酶解，构建用于本研究样本SWATH-MS无标记定量分析的谱图库。
不同体液所含蛋白质的谱图库构建完成后，对各类体液的定量谱分别进行个体化鉴定。
本数据集涵盖蛋白质区域原始数据、谱图库、谱图库检索结果及谱图库蛋白质鉴定结果相关数据。