Hepatic gene expression profiles after long-term high-fat diet feeding in wild type and Nrf2 knock-out mice.. Mus musculus

To assess the role of the transcription factor NFE2 related factor 2 like 2 ( Nrf2) in the development of high-fat diet (HFD)-induced obesity and non-alcoholic HFD-induced fatty liver disease, 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) C57BL6J male mice (obtained from Riken BRC, Tsukuba, Japan and originally developed by Prof. M. Yamamoto) were fed an HFD (60 kcal % fat) for 180 days. Whole genome microarray expression profiling was performed in pooled liver samples of WT and Nrf2-KO mice to identify genes that are differentially expressed between WT and Nrf2-KO mice under the stress conditions of HFD-induced obesity. Overall design: Liver samples were taken from 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) male mice on high-fat diet (60 kcal % fat) for 180 days. Total RNA was isolated from pooled liver samples from WT or Nrf2-KO mice to produce 4 samples each using the guanidinium thiocyanate method.

为探究转录因子NFE2相关因子2样蛋白2（NFE2 related factor 2 like 2, Nrf2）在高脂饮食（high-fat diet, HFD）诱导肥胖及非酒精性高脂饮食性脂肪肝疾病发生发展中的作用，本研究选取8只野生型（wild type, WT）与8只Nrf2基因敲除（Nrf2 knock-out, Nrf2-KO）的C57BL6J雄性小鼠（由日本筑波理化学研究所生物资源中心Riken BRC提供，最初由M. Yamamoto教授构建），以60 kcal%脂肪含量的高脂饲料喂养180天。为鉴定高脂饮食诱导肥胖应激状态下野生型与Nrf2基因敲除小鼠间的差异表达基因，研究人员对两组小鼠的混合肝脏样本开展全基因组微阵列表达谱分析（whole genome microarray expression profiling）。实验整体设计：采集经60 kcal%脂肪含量高脂饲料喂养180天的8只野生型与8只Nrf2基因敲除雄性小鼠的肝脏样本，采用硫氰酸胍法（guanidinium thiocyanate method）分别从野生型和Nrf2基因敲除小鼠的混合肝脏样本中提取总RNA，每组制备4个样本。