Human T-cell dysfunction induced in repeatedly stimulated cultures.

We report the changes in mRNA profile of quiescent, activated and dysfunctional human CD8+ T cells in culture according to a protocol of weekly polyclonal stimulations relevant to immunotherapy. mRNA profile of quiescent enriched CD8+ T cells compared to cells after one stimulation with anti-CD3/CD38 beads or 5 to 7 rounds of weekly stimulations.本研究报道了基于免疫治疗相关的每周多克隆刺激培养方案，体外培养的静息、活化及功能失调的人CD8+ T细胞的mRNA表达谱（mRNA profile）变化。本研究对比了富集静息状态的人CD8+ T细胞，与经抗CD3/抗CD38磁珠（anti-CD3/CD38 beads）单次刺激后的细胞，以及经过5至7轮每周刺激后的细胞的mRNA表达谱。