Supplement S1 - Krüppel Like Factor 4 Promoter Undergoes Active Demethylation during Monocyte/Macrophage Differentiation

Supplement S1 contains primer sequences used for A) methylation specific qPCR B) Primers used for sequencing bisulfite converted genomic DNA C) Primers used for methylation specific PCR of GATA2 pomoter D) 5′ biotin labelled 76 bp KLF4 promoter oligo used for EMSA E) Primers used for cloning KLF4 promoter F) Primers used for KLF4 expression G) Primers used for AICDA expression H) Sequencing and confirmation of AICDA transcript from differentiating PU/ER(T) cells.
(DOCX)

补充材料S1包含如下引物序列及相关实验内容：A) 用于甲基化特异性定量PCR（methylation specific qPCR）的引物；B) 用于亚硫酸氢盐转化基因组DNA测序的引物；C) 用于GATA2启动子甲基化特异性PCR的引物；D) 用于电泳迁移率变动分析（EMSA）的5'端生物素标记的76 bp KLF4启动子寡核苷酸链；E) 用于克隆KLF4启动子的引物；F) 用于KLF4基因表达检测的引物；G) 用于AICDA基因表达检测的引物；H) 对经诱导分化的PU/ER(T)细胞中的AICDA转录本进行测序与验证。（DOCX格式）