Effect of miR17-92 and miR-106b-25 loss in primary calvarial cells. Effect of miR17-92 and miR-106b-25 loss in primary calvarial cells

Gene expression profiling of primary calvarial cells with miR-17-92:miR-106b-25 conditional deletion Overall design: 10-day-old primary calvarial cells of floxed miR-17-92(fl/fl):miR-106b-25(-/-) mice were isolated with collagenase digestion, cultured in alpha-MEM containing 10% fetal bovine serum, and infected with adenoviruses expressing Cre or YFP. 3 days after adenovirus infection, RNA was extracted with Trizol, purified with Zymo spin columns, and subjected to RNA-seq

miR-17-92:miR-106b-25条件性敲除原代颅盖骨细胞的基因表达谱分析
实验设计概述：取10日龄miR-17-92位点loxP侧翼纯合（fl/fl）且miR-106b-25基因敲除（-/-）小鼠的原代颅盖骨细胞，经胶原酶消化法分离后，置于含10%胎牛血清的α-MEM培养基中培养，再用表达Cre重组酶或黄色荧光蛋白（Yellow Fluorescent Protein，YFP）的腺病毒感染细胞。腺病毒感染3天后，采用Trizol法提取RNA，经Zymo离心柱纯化后进行RNA测序（RNA-seq）。