The effect of PGC-1α adenovirus on gene expression in mouse primary hepatocytes

The effect of PGC-1α overexpression using adenovirus (PGC-1α-Ad) on hepatic gene expression was studied in mice primary hepatocytes. The effect of PGC-1α-Ad was studied in male 9-week-old C57BL/6 mouse primary hepatocytes. Cells were treated with PGC-1α-Ad (MOI 1) for 36 h, GFP-Ad (MOI 1) was used as a control. Three parallel RNA samples were pooled in both groups. DNA microarrays were used for gene expression profiling.

本研究以9周龄雄性C57BL/6小鼠的原代肝细胞为实验模型，探究了腺病毒介导的过氧化物酶体增殖物激活受体γ辅激活因子1α（PGC-1α）过表达载体（PGC-1α-Ad）对肝脏基因表达的调控作用。实验过程中，细胞以感染复数（Multiplicity of Infection, MOI）为1的PGC-1α-Ad处理36小时，同时以同等感染复数条件下的绿色荧光蛋白腺病毒（GFP-Ad）作为空白对照。两组均收集三份平行RNA样本并混合后用于后续检测，最终采用DNA微阵列技术完成基因表达谱分析。