the myoblast differentiation difference in normal and DM1 murine cell models

To study the gene expression profile difference in the myoblast differentiation of normal and DM1 groups, we performed RNA-seq on the total RNA samples collected from the in vitro myoblast differentiation day 4 of normal and DM1 C2C12 cell models. Normal and DM1 cell models were bulit by stably transfecting C2C12 cells with GFP-CUG5 and GFP-CUG200 plasmids. Each group contained three biological replicates. The expression matrix was obtained by Hisat2 followed by Stringtie. RNA-seq of normal and DM1 C2C12 cell differentiated for 4 days

为探究正常组与DM1组成肌细胞分化过程中的基因表达谱差异，我们针对正常及DM1型C2C12细胞模型体外成肌分化第4天收集的总RNA样本开展了RNA测序（RNA-seq）。正常与DM1细胞模型通过将C2C12细胞稳定转染GFP-CUG5与GFP-CUG200质粒构建获得。每组设置3个生物学重复。表达矩阵经Hisat2处理后通过Stringtie分析获取。本数据集涵盖正常与DM1型C2C12细胞体外分化4天后的RNA测序数据。