MicroRNAs among esophageal cancer cell sublines with different migration ability. Homo sapiens

Cellular directed migration is critical to invasion-metastasis cascade of cancer cells. We used in vitro transwell model to screen two esophageal cancer cell lines (KYSE30/180) and obtained two pairs of subpopulations with distinc motiliyt ability. Then we used microarrays to detail the differentially expressed genes and microRNAs between these two cell sublines (30U/D and 180U/D) to identify those responsible for ESCC motility. Overall design: KYSE30/180 cells were subject to four successive selections using transwell (CORNING, USA). Subpopulations penetrated through membrane (D) or not (U) were harvested respectively for RNA extraction and hybridization on Affymetrix genome and LC Sciences microRNA microarrays

细胞定向迁移对于癌细胞的侵袭-转移级联反应至关重要。本研究采用体外Transwell（透化小室）模型筛选两株食管癌细胞系（KYSE30/180），获得两对具有显著迁移能力差异的细胞亚群。随后通过芯片技术对这两株细胞亚系（30U/D与180U/D）间的差异表达基因与微小RNA（microRNA）进行全景式分析，以筛选出与食管鳞状细胞癌（ESCC）迁移相关的调控因子。实验整体设计：对KYSE30/180细胞采用美国康宁（CORNING）公司的Transwell小室进行连续四轮筛选，分别收集穿透膜层的细胞亚群（D组）与未穿透膜层的细胞亚群（U组），提取RNA后分别在Affymetrix全基因组芯片与LC Sciences公司的微小RNA芯片上完成杂交检测。