Single cell RNAseq analysis of mouse AAA samples

We report the application of single-cell-based RNA sequencing technology for high-throughput profiling of mice abdominal aortic aneurysm cell type dependent transcriptome. This study provides insight in the expression profile of aortic tissue macrophages in pathological conditions related to cardiovascular diseases. Overall design: Examination of cell specific transcriptomes in three pooled AAA single cell suspensions from three pooled Apolipoprotein deficient mice perfused for 28 days with angiotensin II

本研究报道了单细胞RNA测序（single-cell RNA sequencing）技术在小鼠腹主动脉瘤（abdominal aortic aneurysm, AAA）细胞类型特异性转录组高通量谱分析中的应用。本研究为阐明心血管疾病相关病理状态下主动脉组织巨噬细胞的表达谱提供了新见解。整体实验设计：对来源于3只经血管紧张素II（angiotensin II）灌流28天的载脂蛋白缺陷小鼠（Apolipoprotein deficient mice）的3份混合腹主动脉瘤单细胞悬液的细胞特异性转录组进行检测分析。