CIL: 50727, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. 7.3_Test_Cell_MATLAB_Volume_Analysis_Results is a compressed (zipped) folder containing all files required to execute the MATLAB volume analysis. The folder also includes the resulting output files and figures. This file is a part of Dataset 7, comprised of files used as a technical validation dataset. It is an artificial dataset with manually placed gold beads, used to access the accuracy of the MATLAB script. The choice of gold bead placement location was unique for each row of stereocilia to control for potential errors of stereocilia placement within the rows during MATLAB analysis. No beads were placed on the surface of the kinocilium.

图像通过FEI Helios聚焦离子束扫描电子显微镜（FEI Helios FIB-SEM）采集，采用背散射探测器，样本为经环氧树脂包埋的内耳柯蒂氏器（Organ of Corti）样品。7.3_Test_Cell_MATLAB_Volume_Analysis_Results 为zip压缩文件夹，内含执行MATLAB体积分析所需的全部文件，同时包含分析得到的输出文件与图像结果。本文件属于数据集7的一部分，该数据集为技术验证数据集，由相关配套文件构成。本数据集为人工植入金微珠的人工数据集，用于评估该MATLAB脚本的分析准确性。金微珠的放置位置针对每一排静纤毛（stereocilia）均做了个性化定制，以控制MATLAB分析过程中静纤毛在排内排布可能产生的潜在误差。未在动纤毛（kinocilium）的表面放置金微珠。