Microbial community composition of long-term wheat-fallow and wheat-pea rotations in the dryland Pacific Northwest

The study objective was to investigate chemical and biological soil properties and the response of microbial communities when a rotation system changes from a simple wheat-fallow to a more diverse wheat-pea system, in the presence or absence of N fertilizers. We sampled soils from a long-term field experiment located near Pendleton, OR, USA. Measured soil properties included ammonium, nitrate, phosphate, total carbon, total nitrogen, total sulfur, active carbon, pH, microbial biomass carbon, and soil enzymes including beta-glucosidase, beta-glucosaminidase, acid phosphatase and arylsulfatase. Microbial community DNA was amplified for 16S V4 and LSU D2 regions and paired-end sequenced on the Illumina MiSeq platform.本研究旨在探究当轮作体系从单一的小麦-休耕模式转变为更具多样性的小麦-豌豆轮作模式时，土壤化学与生物学特性及微生物群落的响应，试验设置氮肥施用与不施用两组对照。供试土壤采自美国俄勒冈州彭德尔顿附近的长期田间定位试验，本次测定的土壤参数包括铵态氮、硝态氮、有效磷、总碳、总氮、总硫、活性碳、pH值、微生物生物量碳，以及β-葡萄糖苷酶、β-氨基葡萄糖苷酶、酸性磷酸酶与芳基硫酸酯酶四类土壤酶活性。随后，研究人员对微生物群落总DNA的16S V4区与核糖体大亚基（Large Subunit, LSU）D2区进行扩增，并在Illumina MiSeq测序平台上完成双端测序。