Expression profiles of the G protein-coupled estrogen receptor (GPER)-knockout versus intact cardiomyocytes. Mus musculus

To determine if there are differences in the gene expression profile of cardiomyocytes with GPER deletion when compared to intact cardiomyocytes in both sexes. None of the gene expression profiles have been previously published. Here we provide the original DNA microarray data with the hope that other investigators will be able to utilize this data in their own research. Overall design: Cardiomyocytes were isolated from adult male and female GPER knockout and wild type mice. Total RNA was isolated from cardiomyocytes and gene expression levels were profiled on the Affymetrix GeneAtlas Mouse MG-430 PM Array Strips.

本研究旨在探究雌雄两性中，G蛋白偶联雌激素受体（GPER）基因缺失心肌细胞与野生型完整心肌细胞的基因表达谱差异。所有基因表达谱数据均未在既往研究中发表。本研究公开原始DNA微阵列（DNA microarray）数据，以期为其他科研人员的相关研究提供应用便利。

实验设计概要：从成年雄性、雌性GPER基因敲除与野生型小鼠中分离心肌细胞；提取心肌细胞总RNA后，采用Affymetrix GeneAtlas小鼠MG-430 PM阵列条带进行基因表达水平谱分析。