Gene expression of clonal subpopulations derived from the 4T1 murine mammary carcinoma cell line. Gene expression of clonal subpopulations derived from the 4T1 murine mammary carcinoma cell line

Breast tumors often exhibit intratumoral heterogeneity. We hypothesized that phenotypically distinct clonal subpopulations in heterogeneous tumors may affect overall tumor morphology and cancer cell invasion. In order to study heterogeneous tumors, we isolated individual cells from a parental 4T1 murine mammary carcinoma cell line and generated four clonal subpopulations (E1, E2, A, and M). To characterize these subpopulations, we examined gene expression by performing bulk RNA-sequencing. Overall design: We performed gene expression analysis on clonal, iRFP-expressing subpopulations derived from a parental population of 4T1 murine mammary carinoma cells. We collected RNA from three biological replicates for each sample.

乳腺肿瘤常表现出瘤内异质性（intratumoral heterogeneity）。本研究假设，异质性肿瘤中表型迥异的克隆亚群可能会影响肿瘤整体形态与癌细胞侵袭能力。为开展异质性肿瘤相关研究，我们从亲本4T1小鼠乳腺癌细胞系中分离得到单个细胞，并构建了4株克隆亚群（E1、E2、A及M）。为表征这些亚群的特征，我们通过批量RNA测序（bulk RNA-sequencing）技术检测基因表达水平。实验设计：我们对源自亲本4T1小鼠乳腺癌细胞系的克隆型、表达iRFP的亚群进行基因表达分析，每个样本设置3次生物学重复并收集其RNA。