m6A modification in cisplatin-resistant cells. m6A modification in cisplatin-resistant cells

We report the application of specific antibodies and high-throughput sequencing technologies (methylated RNA immunoprecipitation sequencing, MeRIP-seq) for high-throughput profiling of m6A modifications in NSCLC cisplatin resistant cells. We generated maps of m6A modified transcripts in A549 and A549/DDP cells. We find that the m6A level was significantly increased in A549/DDP cells compared to the A549 cells. We show that there was a close correlation between the m6A modification and cisplatin resistance. Overall design: Examination of m6A modifications in 2 cell types

本研究报道了特异性抗体与高通量测序技术——甲基化RNA免疫沉淀测序（methylated RNA immunoprecipitation sequencing，MeRIP-seq）——在非小细胞肺癌（non-small cell lung cancer，NSCLC）顺铂耐药细胞中开展N6-甲基腺嘌呤（m6A）修饰高通量谱分析的应用。我们构建了A549细胞与A549/DDP细胞中m6A修饰转录本的修饰图谱。研究发现，相较于A549细胞，A549/DDP细胞中的m6A修饰水平显著升高。结果表明，m6A修饰与顺铂耐药性之间存在紧密关联。整体实验设计：对2种细胞类型中的m6A修饰进行检测。