Database of genotypes of significant SNPs of samples of the 2nd trial (A23) of sheep breeds in Latvia within the framework of the project of the Latvian Council of Science LZP-2021/1-0489

Project of The Latvian Council of Science (LCS) - LZP-2021/1-0489 project: “Development of an innovative approach to identify biological determinants involved in the between-animal variation in feed efficiency in sheep farming”. The aim of the project is to determine whether the feed efficiency status of Latvian meat sheep breeds could be predicted using a panel of genetic and molecular markers previously found to be associated with divergent FE status in a training population of lambs when fed the same diet. Novelty: to determine the parameters predicting the most productive result of lamb rearing, we set out to develop the cheapest and most effective method for determining markers of feed efficiency - based on molecular and genetic markers obtained from the blood of live lambs. About the project: The costs associated with lambing (buying or keeping sheep) and preparing or purchasing feed are the two most significant components of variable costs in sheep raising. Feed costs are high due to poor grain growing conditions in major producing countries, the use of feed grains in ethanol production, and increased competition for land in crop production compared to urban development. Feed efficiency in growing lambs (i.e., the animal’s ability to reach a market or adult body weight (BW) with the least feed intake) is a critical factor in the sheep industry. Improving FE reduces production costs. Improving FE by 5% can bring economic benefits up to four times higher than a 5% increase in average daily gain (ADG). Traditionally, meat breeding programs have focused on outputs due mainly to the routine availability of phenotypic data on outputs or correlated traits. Currently, no marker has successfully explained enough of the variability in feed efficiency that they were used as part of a routine improvement program. According to our data, no genetic parameters for performance and feed efficiency traits are available for sheep. The physiological determinants of feed efficiency or putative biomarkers used to analyse animal-to-animal variation in live lambs could be a cost-effective and rapid tool for genetic selection or management decisions. About the data of the project: LZP-2021/1-0489 project data on lamb samples of the second year: 2023, or A23 group, which consists of 92 intensively fattened lambs from eight breeds, including LT breed, which were raised in the meadow and are semi-sibi lambs within the scope of the study. The database contains genotyping results for 57 SNPs, for which a statistically significant association with one of the feed digestion indicators was found in the project's first group (A22). A laboratory-based genotyping method was developed for each selected SNP, which was tested using samples from the A22 group; the results were obtained using the NGS methodology. In the database, SNPs are coded with a laboratory code and an rs ID number. The gene in which the SNP is located is also indicated. The genotypes of each SNP are presented using standard IUB/IUPAC nucleic acid codes, representing the genotype of two alleles at a single locus with a single letter. The data is the joint property of the participants of the LCS project: the University of Latvia and the Latvian University of Life Sciences and Technologies.拉脱维亚科学委员会（Latvian Council of Science, LCS）LZP-2021/1-0489号项目：“绵羊养殖中饲料效率个体间差异相关生物学决定因子的创新性鉴定方法开发”。 本项目旨在验证：针对饲喂同一日粮的羔羊训练群体中已被证实与饲料效率（Feed Efficiency, FE）分化表型相关的一组遗传与分子标记，是否可用于预测拉脱维亚肉用绵羊品种的饲料效率状态。 本项目的创新之处在于：为确定可预测羔羊育肥最优生产性能的参数，我们拟开发一套基于活体羔羊血液提取的分子与遗传标记的、兼具成本效益与高效性的饲料效率标记鉴定方法。 关于本项目： 羔羊繁育（购羊或饲羊）与饲料制备/采购的成本，是绵羊养殖可变成本中占比最高的两大组成部分。受主产国谷物种植条件不佳、燃料乙醇产业对饲料谷物的消耗，以及作物生产用地与城市开发用地竞争加剧等因素影响，饲料成本居高不下。育肥羔羊的饲料效率，即动物以最低采食量达到商品体重或成年体重的能力，是绵羊养殖业的核心影响因素。提升饲料效率可有效降低生产成本：饲料效率每提升5%所带来的经济效益，最高可达平均日增重（Average Daily Gain, ADG）提升5%的四倍。 传统肉用家畜育种项目多聚焦于生产性状，主要原因在于生产性状或相关性状的表型数据可常规获取。目前尚无任何标记能够充分解释饲料效率的个体间变异，以应用于常规育种改良项目。据现有研究资料，尚无针对绵羊生产性能与饲料效率性状的遗传参数公开报道。可用于分析活体羔羊个体间饲料效率差异的生理学决定因子或潜在生物标志物，有望成为用于遗传选育或管理决策的低成本、快速检测工具。 关于本项目数据集： LZP-2021/1-0489项目2023年度（即A23组）羔羊样本数据：该组包含来自8个品种的92只集约育肥羔羊，其中包含LT品种，所有受试羔羊均在牧场饲养，且为本研究范围内的半同胞羔羊。 本数据库包含57个单核苷酸多态性（Single Nucleotide Polymorphism, SNP）的基因分型结果，上述SNP在项目第一组（A22组）样本中被证实与至少一项饲料消化指标存在统计学显著关联。本项目为每个筛选出的SNP开发了实验室分型方法，并通过A22组样本完成了方法验证，分型结果采用下一代测序（Next-Generation Sequencing, NGS）技术获取。 数据库中，SNP以实验室代码与rs编号进行编码，同时标注其所在基因。每个SNP的基因型采用标准IUB/IUPAC核酸编码格式表示，即以单个字母代表单个基因座上的两个等位基因的基因型。 本数据为拉脱维亚大学与拉脱维亚生命科学与技术大学参与的LCS项目参与方的共同财产。