Platelet-specific TGF beta1 deficiency aggravates atherosclerosis, vascular inflammation, and hypercholesterolemia in mice

Atherosclerosis involves inflammatory and thrombotic mechanisms, to which both platelets and transforming growth factor beta contribute. The effect of platelet-derived TGF beta on atherosclerosis is, however, unknown and therefore investigated. Murine platelet-selective TGF beta-deficiency (plt-TGF beta-/-) was created by a Pf4-Cre approach, and an atherosclerotic mouse model was established by functional abrogation of Ldlr and 10-15 weeks of a high-fat diet in plt-TGF beta-/- mice and their non-plt-TGF beta-/- littermates.Total RNA of the aorta was isolated according to the manufacturers instructions,the library was constructed according to the BGI Genomics.

动脉粥样硬化（Atherosclerosis）涉及炎症与血栓形成机制，血小板与转化生长因子β（transforming growth factor beta, TGF-β）均参与该病理过程。然而，血小板源性TGF-β对动脉粥样硬化的影响尚未明确，因此本研究针对该问题开展探究。研究通过Pf4-Cre重组酶系统构建了血小板选择性TGFβ缺陷小鼠（plt-TGFβ-/-）；并在plt-TGFβ-/-小鼠及其非血小板选择性TGFβ缺陷同窝对照小鼠中，通过敲除低密度脂蛋白受体（Ldlr）功能并施加10~15周高脂饮食，成功建立动脉粥样硬化小鼠模型。按照制造商说明书提取主动脉总RNA，并参照华大基因（BGI Genomics）的建库流程构建测序文库。