Immune Isoform Atlas: Landscape of alternative splicing in human immune cells

This dataset contains the global and terminal proteome of THP-1 and LCL cell lines untreated or treated with phorbol 12-myristate 13-acetate (PMA) and interferon-α2 (IFN-α2), respectively. Extracted proteins were digested with trypsin or V8 protease and protein terminal peptides were isolated with strong cation exchange chromatography or metal oxide-based ligand-exchange chromatography. All peptides were labeled with TMT-tag, and a total of 16 channels were used with quadruplicates of untreated LCL, IFN-α2-treated LCL, untreated THP-1, and PMA-treated THP-1 peptides, respectively. The labeled peptides were fractionated into 24 vials by reversed-phase HPLC at high pH conditions.

本数据集涵盖未经处理或分别经佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）与干扰素α2（IFN-α2）处理的THP-1细胞系与LCL细胞系的全局蛋白质组及末端蛋白质组。提取的蛋白质经胰蛋白酶或V8蛋白酶酶解后，通过强阳离子交换色谱法或金属氧化物基配体交换色谱法分离得到蛋白质末端肽段；所有肽段均采用TMT标签（TMT-tag）标记，共启用16个标记通道，分别为未经处理的LCL、经IFN-α2处理的LCL、未经处理的THP-1以及经PMA处理的THP-1肽段设置四重重复；标记后的肽段在高pH条件下经反相高效液相色谱分离为24个组分瓶。