PMACS-103

<br><b>Project title</b>: PMACS-103<b>Grant Information:</b> AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarction<b>Project Description:</b>· Animal Model: Myocardial Infarction· Animal Age: 8-12 Weeks· Animal Sex: Male· Animal Strain: C57B6/J Lys^CreGATA6^fl/fl· Number: 8 Mice· Groups: All mice given 100uL of CFA emulsion near right hip joint and then infarcted· Experimental Conditions: Mice were given 100uL of CFA emulsion near the right hip joint, injected subcutaneously. At day 7 post-injection, mice were given MI via LAD ligation. Animals were sacrificed at day 3 post-infarction. Hearts were collected and analyzed via Flow Cytometry. Results were compared to cohort from PMACS-102 for analysis<b>Code/Scripts: </b>N/A<b>Licenses: </b>N/A

<b>项目标题</b>：PMACS-103<br><b>资助信息</b>：美国心脏协会（American Heart Association, AHA）资助项目编号9TPA34910007，研究主题为「心包巨噬细胞在心肌梗死中的调控作用」<br><b>项目描述</b>：· 动物模型：心肌梗死模型<br>· 动物周龄：8~12周<br>· 动物性别：雄性<br>· 动物品系：C57B6/J Lys^CreGATA6^fl/fl<br>· 小鼠数量：8只<br>· 实验分组：所有小鼠均于右侧髋关节附近皮下注射100μL完全弗氏佐剂（Complete Freund's Adjuvant, CFA）乳剂，随后构建心肌梗死模型<br>· 实验流程：小鼠皮下注射右侧髋关节附近100μL CFA乳剂，于注射后第7天通过左前降支（Left Anterior Descending, LAD）结扎构建心肌梗死模型；于梗死造模后第3天处死动物，收集心脏并通过流式细胞术（Flow Cytometry）进行分析，所得结果将与PMACS-102队列进行对比分析<br><b>代码/脚本</b>：无<br><b>许可协议</b>：无