Identification of elevated A-to-I editing sites due to expression of an active ADAR3 mutant in human glioblastoma cells

We have analyzed RNA-seq data to identify A-to-I editing sites in two groups of samples: one group isolated from human U87 cell line expressing an active ADAR3 mutant while the other isolated from U87 cell line expressing the inactive counterpart of the ADAR3 mutant. We compared these two groups of samples and identified sites whose editing levels are higher in the first group than in the second group. Examine A-to-I editing sites in two group of samples.

我们通过对RNA测序（RNA-seq）数据开展分析，在两组样本中鉴定A-to-I编辑位点（A-to-I editing sites）：一组样本分离自表达活性ADAR3突变体的人U87细胞系，另一组分离自表达该ADAR3突变体无活性变体的U87细胞系。我们对这两组样本进行比较分析，筛选出第一组中编辑水平高于第二组的编辑位点，并对两组样本中的A-to-I编辑位点进行检测。