Supplemental video 6.C. BCG-hMCs interactions are increased by IL-33 pre-treatment

hMCs were treated for 24h with IL-33 (50ng/ml) or left unstimulated (control). After incubation cells were washed and left 30min in an 8 well chamber at 37C. BCG mCherry at a MOI 10:1 was added to wells and a video was recorded for 8h using confocal microscopy at 37C and 5% CO2 with 30-second interval acquisitions. Videos were analysed using FIJI and compressed to 4 minutes to allow visualization. Video 1 shows naïve MCs and Video 2 shows IL-33 pre-treated MCs. Videos are representative of 2 independent experiments

将人类肥大细胞（human mast cells, hMCs）以浓度为50ng/ml的白介素33（Interleukin-33, IL-33）处理24小时，同时设置未受刺激的对照组。孵育结束后洗涤细胞，将其接种于8孔腔室中并于37℃静置30分钟。随后向各孔加入感染复数（multiplicity of infection, MOI）为10:1的mCherry标记卡介苗（Bacillus Calmette-Guérin, BCG mCherry），并在37℃、5% CO₂环境下采用共聚焦显微镜以30秒的采集间隔连续录制8小时的延时视频。采用FIJI软件对所得视频进行分析，并将视频压缩至4分钟以方便可视化展示。视频1展示未预处理的肥大细胞，视频2展示经IL-33预处理的肥大细胞；所有视频均为2次独立重复实验的代表性样本。