Adipose tissue from metabolic syndrome mice induces an aberrant miRNA signature highly relevant in prostate cancer development

We developed a MeS-like disease model using C57BL/6J mice chronically fed with high fat diet (HFD) that were inoculated with TRAMP-C1 prostate cancer (PCa) cells to investigate the interaction between white adipose tissue (WAT) and PCa cells through miRNAs, in MeS mice. In this dataset, we include the expression data obtained from co-culture medium between TRAMP-C1 cells with HFD-gonadal WAT (gWAT) versus TRAMP-C1 cells with CD-gWAT. For differential expression analysis we used the Rank Product Method. TRAMP-C1 cells were co-cultured in 24-transwell plates with gWAT obtained from CD or HFD C57BL/6J mice. Culture medium was collected for RNA isolation followed by miRNA microarrays hybridization. As a control, TRAMP-C1 cells were cultured without the addition of gWAT.本研究构建了代谢综合征（Metabolic Syndrome, MeS）样疾病模型：以长期饲喂高脂饮食（high fat diet, HFD）的C57BL/6J小鼠为受试对象，接种TRAMP-C1前列腺癌细胞（prostate cancer, PCa），以探究代谢综合征小鼠体内白色脂肪组织（white adipose tissue, WAT）与前列腺癌细胞通过微小RNA（microRNAs, miRNAs）介导的相互作用。本数据集包含两组共培养体系的表达谱数据：一组为TRAMP-C1细胞与高脂饮食喂养小鼠的性腺白色脂肪组织（gonadal WAT, gWAT）共培养，另一组为TRAMP-C1细胞与常规饮食（chow diet, CD）喂养小鼠的性腺白色脂肪组织共培养。本研究采用秩积法（Rank Product Method）进行差异表达分析。实验中，将TRAMP-C1细胞与取自常规饮食或高脂饮食C57BL/6J小鼠的性腺白色脂肪组织置于24孔Transwell小室培养板中共培养，收集培养上清液用于RNA提取，随后进行miRNA芯片杂交。作为对照，本研究设置了仅单独培养TRAMP-C1细胞、未添加性腺白色脂肪组织的对照组。