RNA-seq for UBR5 and Cdc73-regulated genes in 4T1 cells treated with Dovitinib. RNA-seq for UBR5 and Cdc73-regulated genes in 4T1 cells treated with Dovitinib

In order to understand the molecular mechanisms underlying the regulatory activities of UBR5 and CDC73 in dovitinib-induced apoptosis in 4T1 tumor cells that were Dox-silenced for the expression of Ubr5, Cdc73, or both and treated with dovitinib. Overall design: Comparative gene expression profiling analysis of RNA-seq data for 4T1 cells and its KD derivatives (shUbr5, shCdc73, and shUbr5-Cdc73)

为阐明UBR5与CDC73在多韦替尼（dovitinib）诱导的4T1肿瘤细胞凋亡过程中调控活性的分子机制，本研究针对经多西环素诱导分别沉默Ubr5、Cdc73或同时沉默二者基因表达，并经多韦替尼处理的4T1肿瘤细胞开展实验。总体实验设计：对4T1野生型细胞及其基因敲低（KD）衍生株（shUbr5、shCdc73及shUbr5-Cdc73）的RNA测序（RNA-seq）数据进行比较基因表达谱分析。