Human bladder smooth muscle cells - effect of stretch in vitro

The aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. Total RNA was extracted from both groups of cells, reverse-transcribed, biotin-labeled, fragmented and hybridized to HG-U133A. Four biological replicates were generated for each treatment group (non-stretched or stretched). Keywords = bladder Keywords = smooth muscle cells Keywords = cyclic stretch-relaxation Keywords: other

本实验旨在探究周期性张弛（cyclic stretch-relaxation，“拉伸”）对正常二倍体人膀胱平滑肌细胞基因表达谱的影响。将细胞接种于硅弹性体底部的6孔培养板中，培养至约80%汇合度后进行48小时血清饥饿处理，随后以20%伸长率施加4小时的周期性张弛刺激。将接种于拉伸培养板但未施加拉伸刺激的细胞作为对照组。提取两组细胞的总RNA，经反转录、生物素标记、片段化后，与HG-U133A基因芯片进行杂交。每个处理组（非拉伸组与拉伸组）均设置4次生物学重复。关键词：膀胱、平滑肌细胞、周期性张弛、其他。