Transcriptome profiling of Panax notoginseng response to arsenic stress and dark septate endophytes (DSE) inoculation

This study aims to investigate the molecular mechanisms underlying the interaction between dark septate endophytes (DSE) and Panax notoginseng under arsenic (As) stress. We conducted a controlled pot experiment with four treatments:(1) As stress alone, and (2) DSE inoculation combined with As stress. Root tissues of Panax notoginseng were collected for RNA extraction. High-throughput RNA sequencing (RNA-seq) was performed to identify differentially expressed genes and key pathways involved in the DSE-mediated alleviation of arsenic toxicity.

本研究旨在探究砷（As）胁迫下暗色有隔内生真菌（dark septate endophytes, DSE）与三七（Panax notoginseng）之间的互作分子机制。本研究设置受控盆栽实验，共包含4组处理：(1) 单独砷胁迫组，(2) 暗色有隔内生真菌接种联合砷胁迫组。采集三七的根组织用于RNA提取，通过高通量RNA测序（RNA-seq）鉴定参与DSE介导的砷毒性缓解过程的差异表达基因与关键通路。