Splicing analysis of Anopheles gambiae Gam1-MM and MM-CP transgenic strains

We augmented two midgut genes of the malaria mosquito Anopheles gambiae to secrete the exogenous antimicrobial peptides Magainin 2 and Melittin, and to enable non-autonomous gene drive via an intronic guide RNA. To assess the splicing efficiency of the artificial intron, we performed sequencing of the cDNA amplicon over the splice site.

我们对疟疾媒介冈比亚按蚊（Anopheles gambiae）的两个中肠基因进行了基因改造，使其能够分泌外源抗菌肽马加宁2（Magainin 2）与蜂毒素（Melittin），并可通过内含子向导RNA（intronic guide RNA）实现非自主基因驱动。为评估该人工内含子（artificial intron）的剪接效率，我们针对剪接位点区域的cDNA扩增子开展了测序分析。