NGS analysis of T-ALL cell lines treated with mTORC1 inhibitor. NGS analysis of T-ALL cell lines treated with mTORC1 inhibitor

We recentlly showed that everolimus, a mTORC1 inhibitor increases reactive oxygen species (ROS) levels, decreases the levels of NADPH and of glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway (PPP), and induces apoptosis of T-ALL cells. Overall design: Jurkat, TALL-1 and CEM cells lines were treated with 10 μM everolimus or drug vehicle after for 24 hours. Then we collected cells and extracted the RNA to perform the RNASEq analysis.

我们此前的研究表明，依维莫司（everolimus）——一种哺乳动物雷帕霉素靶蛋白复合物1（mTORC1）抑制剂——可升高活性氧（ROS）水平，降低烟酰胺腺嘌呤二核苷酸磷酸（NADPH）与磷酸戊糖途径（PPP）的限速酶葡萄糖-6-磷酸脱氢酶（G6PD）的表达水平，并诱导T细胞急性淋巴细胞白血病（T-ALL）细胞发生凋亡。总体实验设计：将Jurkat、TALL-1及CEM细胞系用10 μM依维莫司或药物溶媒处理24小时，随后收集细胞并提取RNA，开展RNA测序（RNA-seq）分析。