Revealing the Immune Response of Sitona callosus to Beauveria bassiana Infection through Integrative Analyses of Transcriptomics and Metabolomics

The legume pest Sitona callosus poses a significant threat to alfalfa growth. Due to its unique life cycle, Beauveria bassiana has emerged as an effective fungus for controlling S. callosus . We conducted a comprehensive analysis of the transcriptome and metabolome of S. callosus infected by B. bassiana. Differential expression of antifungal genes, including heat shock genes, cytochrome P450 (CYP450) genes, cathepsin proteases, and C-type lectin were identified in S. callosus. Pathway analysis revealed associations between immune genes and metabolites involved in autophagy-animal, glucagon signaling pathway, and glycerophospholipid metabolism. These findings provide valuable insights for enhancing the control efficacy of Beauveria bassiana on Sitona callosus. Overall design: In order to determine the immune response of Sitona callosus infected by Beauveria bassiana. Transcriptomic tests were performed on Sitona callosus after infection with Beauversiana bassiana and the uninfected one.

豆科害虫苜蓿象甲（Sitona callosus）对苜蓿生长构成严重威胁。球孢白僵菌（Beauveria bassiana）凭借其独特的生命周期，成为防控苜蓿象甲的高效生防真菌。本研究针对被球孢白僵菌侵染的苜蓿象甲，开展了转录组（transcriptome）与代谢组（metabolome）的全面联合分析。研究人员在该虫体内鉴定出一批抗真菌基因的差异表达，涵盖热激基因、细胞色素P450（CYP450）基因、组织蛋白酶蛋白酶以及C型凝集素等类别。通路富集分析显示，免疫基因与相关代谢物分别参与了动物自噬、胰高血糖素信号通路以及甘油磷脂代谢过程。本研究结果为提升球孢白僵菌对苜蓿象甲的防控效能提供了极具价值的理论依据。试验设计：为明确球孢白僵菌侵染后苜蓿象甲的免疫应答机制，本研究分别对侵染组与未侵染组的苜蓿象甲实施了转录组检测分析。