MSL-1(Q4453).S2

modENCODE_submission_3293 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody MSL-1(Q4453) (target is MSL-1)

modENCODE_submission_3293：本次提交源自Gary Karpen牵头的modENCODE项目。若需查看modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。
项目目标：本项目旨在定位黑腹果蝇（Drosophila melanogaster）基因组内125种染色体蛋白质的结合位点。本次研究涉及的蛋白质参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。本研究将采用基因组平铺芯片技术结合染色质免疫共沉淀（Chromatin ImmunoPrecipitation, ChIP）实验方法。初期将利用3种细胞系与2个胚胎阶段的染色质完成定位检测，后续将针对部分蛋白质，将分析范围拓展至另外4种动物组织/发育阶段。
有关数据使用的条款与细则，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验类型：ChIP-chip。
生物来源：细胞系：S2-DRSC；组织类型：胚胎来源细胞系；发育阶段：晚期胚胎阶段；性别：雄性；
生物学重复次数：4次；
实验因素：细胞系S2-DRSC；抗体：MSL-1(Q4453)（靶标为MSL-1）