Arabidopsis sepal live-imaging data sets CAT2 and oryzalin
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CAT2 live-imaging data set: These are csv files generated from meshes for various attributes of the cells. The naming system for the files is as follows: Genotype_Replicate_Time Interval_Measure The genotypes are wild type (wt), ftsh4-5 (ftsh4), CAT2oe (CAT2), and ftsh4-5 CAT2oe (ftsh4CAT2). Replicates are numbered 1-3. Time points are named so that t0 is 0hrs, t1 is 24hrs, t2 is 48hrs, and t3 is 72hrs. Two times in the file name indicates the time interval. Oryzalin live-imaging data set: These are csv files generated from meshes for various attributes of the cells. The naming system for the files is as follows: Genotype_Treatment_Replicate_Time Interval_Measure The genotypes are wild type (wt), ftsh4-5 (ftsh4), CAT2oe (CAT2), and ftsh4-5 CAT2oe (ftsh4CAT2). The treatments are mock and oryzalin (ory). Replicates are numbered 1-3 for mock and 1-4 for oryzalin. Time points are named so that t0 is 0hrs, t1 is 24hrs, t2 is 48hrs, and t3 is 72hrs. Two times in the file name indicates the time interval. Fig 4 3-AT treatment microtubules images: These are images of the microtubules from the 3-AT treatment from Fig 4 and S5. It is only the signal from the top of the epidermal cells, and underlying signal was removed using the annihilate function in MorphoGraphX. Files are named as genotype_treatment_replicate. Microtubule screenshots: These are images of the microtubules from the CAT2oe live-imaging from Fig 2 and S2. It is only the signal from the top of the epidermal cells, and underlying signal was removed using the annihilate function in MorphoGraphX. Files are grouped into folders with time interval t0 and t1, and t2 and t3. Files are named as genotype_replicate_time interval. Propyzamide Figure 5 and S6: These are images of the microtubules from the propyzamide experiment from Fig 5 and S6. It is only the signal from the top of the epidermal cells, and underlying signal was removed using the annihilate function in MorphoGraphX. Files are named as date_genotype_replicate_time point. The options for ‘time point’ are before, which is before treatment, and prop, which is after propyzamide treatment. Tubulin6 microtubule marker: These are images of the microtubules from plants the the GFP-TUB6 marker from Fig S3. It is only the signal from the top of the epidermal cells, and underlying signal was removed using the annihilate function in MorphoGraphX. Files are named as genotype_parent insertion line-replicate. Live imaging meshes: These folders contain the MorphographX meshes and MophographX stacks of the microtubule signal for a given replicate from live -imaging (Fig 1 CAT2oe data set). ftsh4 oryzalin: This folder contains the MorphoGraphX meshes and MophographX stacks of the microtubule signal for the ftsh4-5 replicates from the oryzalin live imaging data set. Folders are labeled ftsh4_treatment_replicate and files are named ftsh4_treatment_replicate_time interval. wt oryzalin: This folder contains the MorphoGraphX meshes MophographX stacks of the microtubule signal for the wild type replicates from the oryzalin live imaging data set. Folders are labeled wt_treatment_replicate and files are named wt_treatment_replicate_time interval.
CAT2 活体成像数据集(CAT2 live-imaging dataset):此类文件为基于细胞各类属性的网格生成的逗号分隔值(CSV)文件。文件命名规则如下: 基因型_重复实验编号_时间间隔_测量指标 基因型包括野生型(wild type, wt)、ftsh4-5(ftsh4)、CAT2oe(CAT2)以及ftsh4-5 CAT2oe(ftsh4CAT2)。重复实验编号范围为1至3。时间点命名规则为:t0对应0小时,t1对应24小时,t2对应48小时,t3对应72小时;文件名中包含两个时间点即代表该时间间隔。 Oryzalin 活体成像数据集:此类文件为基于细胞各类属性的网格生成的逗号分隔值(CSV)文件。文件命名规则如下: 基因型_处理方式_重复实验编号_时间间隔_测量指标 基因型包括野生型(wild type, wt)、ftsh4-5(ftsh4)、CAT2oe(CAT2)以及ftsh4-5 CAT2oe(ftsh4CAT2)。处理方式包括空白对照(mock)与拿草特(Oryzalin, ory)。空白对照组的重复实验编号为1至3,拿草特处理组的重复实验编号为1至4。时间点命名规则与前述一致,文件名中包含两个时间点即代表该时间间隔。 图4及补充图S5的3-氨基-1,2,4-三唑(3-AT)处理微管图像数据集:此类图像取自图4与补充图S5中3-AT处理样本的微管信号,仅保留表皮细胞顶层的信号,并通过MorphoGraphX软件的annihilate功能去除底层冗余信号。文件命名格式为:基因型_处理方式_重复实验编号。 微管截图数据集:此类图像取自图2与补充图S2中CAT2oe活体成像样本的微管信号,仅保留表皮细胞顶层的信号,并通过MorphoGraphX软件的annihilate功能去除底层冗余信号。文件按时间间隔分为t0与t1、t2与t3两个文件夹,命名格式为:基因型_重复实验编号_时间间隔。 氟草胺(Propyzamide)图5及补充图S6数据集:此类图像取自图5与补充图S6中氟草胺处理实验的微管信号,仅保留表皮细胞顶层的信号,并通过MorphoGraphX软件的annihilate功能去除底层冗余信号。文件命名格式为:日期_基因型_重复实验编号_时间点。时间点选项包括处理前(before)与氟草胺处理后(propyzamide, prop)。 微管蛋白6(Tubulin6)标记数据集:此类图像取自补充图S3中携带绿色荧光蛋白-微管蛋白6(GFP-TUB6)标记的植物样本的微管信号,仅保留表皮细胞顶层的信号,并通过MorphoGraphX软件的annihilate功能去除底层冗余信号。文件命名格式为:基因型_亲本插入株系-重复实验编号。 活体成像网格数据集:此类文件夹包含图1中CAT2oe数据集活体成像的指定重复实验样本的微管信号的MorphoGraphX网格与MorphoGraphX堆叠文件。 ftsh4 拿草特处理数据集:该文件夹包含拿草特活体成像数据集中ftsh4-5样本重复实验的微管信号的MorphoGraphX网格与MorphoGraphX堆叠文件。文件夹命名格式为:ftsh4_处理方式_重复实验编号,文件命名格式为:ftsh4_处理方式_重复实验编号_时间间隔。 野生型 拿草特处理数据集:该文件夹包含拿草特活体成像数据集中野生型样本重复实验的微管信号的MorphoGraphX网格与MorphoGraphX堆叠文件。文件夹命名格式为:wt_处理方式_重复实验编号,文件命名格式为:wt_处理方式_重复实验编号_时间间隔。



