16s rRNA of gut microbiome in PBC and pSS

This study aimed to analyze gut microbiome similarities and differences between primary biliary cholangitis (PBC) and primary Sjögren’s syndrome (pSS), exploring potential associations with disease pathogenesis. High-throughput sequencing of the 16S rRNA gene was performed on feacal samples from 100 subjects (PBC: 38, pSS: 42, HC: 20) to compare composition, diversity, and key microbial markers, examining associations with clinical indicators.本研究旨在分析原发性胆汁性胆管炎（primary biliary cholangitis, PBC）与原发性干燥综合征（primary Sjögren’s syndrome, pSS）患者的肠道微生物组异同，并探讨其与疾病发病机制的潜在关联。本研究对100名受试者的粪便样本进行16S rRNA基因高通量测序，其中原发性胆汁性胆管炎组38例、原发性干燥综合征组42例、健康对照组（Healthy Control, HC）20例，以比较菌群组成、多样性及关键微生物标志物，并分析其与临床指标的相关性。